Background: Upper gastrointestinal (GI) endoscopy is a common diagnostic procedure that plays central role in the practice of gastroenterology. It remains the first line of diagnostic modality for evaluation of Gastrointestinal (GI) disorders. It is a safe, commonly used investigation for the evaluation of wide range of upper GI lesions. Aim of this study is to evaluate the effect of information bundle on physiological parameters among upper Gastrointestinal endoscopy patients. Method: Two group pretest posttest research design was used. Sample was selected through convenient purposive sampling technique and total 150 samples were taken into study, equally divided in experimental and control group. Findings: The majority of the patients were from age group 39 years and above. The post intervention means cores showed an improvement in the physiological parameters in experimental group and calculated ‘t’ values were statistically significant at 0.05 level of significance(p<0.05). Conclusion: The Information Bundle was found effective in stabilizing the physiological parameters of patients during endoscopy.